• November 26, 2021
Biovision Rnase-R

RNase R from BioVision

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DESCRIPTION:

RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species. This enzyme does not digest spherical, lariat, or double stranded RNA with temporary 3’ overhangs (decrease than sevennucleotides). As such, this enzyme is ideally suited to the look at of lariat RNA produced by typical splicing, along with circRNAs which come up via back-splicing. By eradicating linear
RNAs from cellular or RNA extracts, RNase R drastically facilitates the identification of spherical species via RNA-sequencing. This allows researchers to probe the panorama of splicing events with greater depth.

APPLICATIONS:

• Enriching circRNAs in natural samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Studying numerous splicing
• Manufacturing of artificial spherical RNAs

EZYME UNIT DEFINITION: One unit is printed as the amount of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.

 

Biovision Rnase-R
Biovision Rnase-R

STORAGE CONDITIONS: Retailer at -20°C.

Avoid repeated freeze-thaw cycles of all elements to retain most effectivity. All elements
are regular for one 12 months from the date of transport when saved and handled accurately.

 

 

Cat # +Dimension M1228-500
Dimension 500 U
Highlights RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species.
Storage Conditions -20°C
Supply Conditions Gel Pack
USAGE For Evaluation Use Solely! Not For Use in Individuals.

SAMPLE PROCESSING GUIDELINES AND TROUBLESHOOTING:

• For digestion of full RNA, longer incubations of 2-Three hours are typically required.
• If degradation is inefficient, use a barely elevated incubation temperature (40-45°C) and complement further enzyme partway (e.g. 0.5 μl after 1 hour) via the method. The higher temperature is very useful for degrading extraordinarily structured linear RNAs, equal to rRNAs. Do not exceed 45°C or incubate over 3
hours, as this can end in non-enzymatic RNA degradation.

• RNase R shows low train on tRNA, rRNA and completely different extraordinarily structured RNAs, for which the three’ end is double stranded with a short 3’ overhang. These RNA species can stall the enzyme and finish in drastically lowered train. If inefficient degradation is seen, it is endorsed to each upscale the digestion, use further RNase R,
or take away rRNA from full RNA extracts earlier to digestion.

• Do not forget that spherical RNAs symbolize a small proportion of full RNA (typically 0.1%-0.01%), as a result of this truth RNase R treatment will most definitely finish in low ranges of RNA (picogram-range), presumably undetectable by most methods. For that motive, a starting amount of not lower than 10 µg of full RNA is helpful for a lot of downstream features.

• Whereas the enzyme could also be heat inactivated the method should not be helpful since extreme heat may end up in RNA hurt. Phenol-chloroform precipitation may be utilized as an alternative. For NGS, sturdy half reversible immobilization (SPRI) bead cleanup is helpful.

• Magnesium at concentrations of 0.1-1.Zero mM is required for optimum train. If ETA is
present, compensate by together with MgCl2 to 1.Zero Mm final focus.

 

 

Histone H3 Peptide (residues 21-44), Biotinylated

R-1006
  • EUR 362.76
  • EUR 119.90
  • EUR 205.70
  • 200 µl
  • 100 ul
  • 200 ul

Histone H4 Peptide (residues 1-21), Biotinylated

R-1007
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H4ac Peptide, Biotinylated

R-1008
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H3K9ac Peptide

R-1009
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H3K9ac Peptide, Biotinylated

R-1010
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H3K14ac Peptide

R-1011
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H3K14ac Peptide, Biotinylated

R-1012
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H4K5ac Peptide

R-1014
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H4K8ac Peptide

R-1015
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H4K12ac Peptide

R-1016
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone Acetyl H4K16ac Peptide

R-1017
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K4me1 Monomethyl Peptide

R-1018
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K4me1 Monomethyl Peptide, Biotinylated

R-1019
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K4me2 Dimethyl Peptide

R-1020
  • EUR 267.06
  • EUR 78.10
  • EUR 140.80
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K4me2 Dimethyl Peptide, Biotinylated

R-1021
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K4me3 Trimethyl Peptide

R-1022
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K4me3 Trimethyl Peptide, Biotinylated

R-1023
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me1 Monomethyl Peptide

R-1024
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me1 Monomethyl Peptide, Biotinylated

R-1025
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me2 Dimethyl Peptide

R-1026
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me2 Dimethyl Peptide, Biotinylated

R-1027
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me3 Trimethyl Peptide

R-1028
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K9me3 Trimethyl Peptide, Biotinylated

R-1029
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me1 Monomethyl Peptide

R-1030
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me1 Monomethyl Peptide, Biotinylated

R-1031
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me2 Dimethyl Peptide

R-1032
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me2 Dimethyl Peptide, Biotinylated

R-1033
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me3 Trimethyl Peptide

R-1034
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K27me3 Trimethyl Peptide, Biotinylated

R-1035
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K36me1 Monomethyl Peptide

R-1036
  • EUR 341.88
  • EUR 106.70
  • EUR 191.40
  • 200 µl
  • 100 ul
  • 200 ul

Histone H3K36me1 Monomethyl Peptide, Biotinylated

R-1037
  • EUR 361.02
  • EUR 119.90
  • EUR 204.60
  • 200 µl
  • 100 ul
  • 200 ul

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