Phire and Phusion Kits
What’s Direct PCR?
Direct PCR (dPCR) is a technique of DNA amplification straight from an animal or plant tissue pattern with out performing DNA isolation and purification steps. This system drastically reduces experimental time, and price in genotyping and high-volume tasks. It additionally supplies a greater possibility when confronted with the challenges of amplifying a really small amount of pattern the place the purification step might doubtlessly result in pattern loss.
In some ways, dPCR works like standard PCR. Each methods depend upon template DNA, primers and a grasp combine or PCR reagents, and each use a thermal cycler for amplification. The first distinction between dPCR and traditional PCR is the tailor-made buffers utilized in dPCR. The modified parts of dPCR allow strong amplification regardless of the presence of PCR inhibitors usually present in crude samples.
Unit Definition
One unit is outlined as the quantity of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance inside 30 min at 74°C.
| Description | Code | Specification | Class | Expertise |
|---|---|---|---|---|
| ALS SARS-CoV-2 RT-PCR Take a look at for surfaces | ALS-B01-Adv | RUO | Meals /Water Virology Detection | Actual-Time PCR |
| ALS SARS-CoV-2 RT-PCR Take a look at for Wastewater | ALS-B02-Adv | RUO | Meals /Water Virology Detection | Actual-Time PCR |
Benefits
- Fast amplification: The amplification fee of the polymerase is 6kb/min. 1kb fragment will be amplified inside 25min.
- Lengthy fragment amplification: For plasmid, λ DNA and different straightforward templates, the polymerase can successfully amplify > 20kb. For genome, the polymerase can successfully amplify > 8kb. And for cDNA, the polymerase can successfully amplify > 8kb.
- Excessive specificity: With scorching begin know-how, the polymerase is 100% inactive beneath 50°C, and might solely be restored by heating at 95°C for 5min.
- Excessive tolerance to impurities: Samples of entire blood, serum, cultured cells and urine will be straight amplified with out prior DNA purification.
ALS SARS-CoV-2 RT-PCR 4G has been developed and produced totally at ALS in Portugal and is designed primarily for export. This new equipment is ready to detect all identified SARS-CoV-2 variants, together with British, Brazilian and South African variants, with out cross-reacting with different identified associated viruses or widespread respiratory pathogens. The selection of the genomic areas used as targets and the sequences of the primers and hydrolysis probes had been primarily based on WHO, CDC and DGS suggestions.
The ALS SARS-CoV-2 RT-PCR 4G equipment is an in vitro diagnostic medical gadget (CE-IVD) that makes use of real-time PCR nucleic acid amplification know-how for the detection of SARS-CoV-2 in medical samples from the higher respiratory tract of people who could or might not be suspected of getting COVID-19.
The equipment makes use of the RT-PCR method, combining amplification of sure areas of the viral genome with its detection by particular hydrolysis probes. The SARS-CoV-2 genome goal areas are extremely conserved and correspond to 2 particular genes: ORF polyprotein (ORF1ab gene) and nucleocapsid phosphoprotein (N gene). The equipment can even detect the gene that codifies the structural protein of the envelope (gene E) particular to the Sarbecovirus subgenus.
amfiSure Direct PCR Lysis Buffer |
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| D0300-010 | GenDepot | 100ml | EUR 165.6 |
Blood Direct PCR Kit V2 |
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| PD103-01 | Vazyme | 50 rxn | EUR 202.8 |
Blood Direct PCR Kit V2 |
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| PD103-02 | Vazyme | 200 rxn | EUR 424.8 |
Mouse Direct PCR Kit (For Genotyping) |
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| B40013 | Bimake | 200 ractions | EUR 250.8 |
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Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication. |
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Mouse Direct PCR Kit (For Genotyping) |
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| B40015 | Bimake | 500 ractions | EUR 490.8 |
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Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication. |
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Mouse Direct PCR Kit (For Genotyping) |
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| B45012 | Bimake | 1ml*5 (500 ractions) | EUR 336 |
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Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication. |
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Mouse Direct PCR Kit (For Genotyping) |
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| B45013 | Bimake | 1ml*20 (2000 ractions) | EUR 1038 |
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Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication. |
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FastAmp Plant Direct PCR Kit - 1250 Reactions |
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| 4615 | Intact Genomics | 1/EA | EUR 621.6 |
Direct RT-PCR Kit wioRealStart DNA Polymerase |
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| FYT507-100P | Yeastern Biotech | 100 Preps | Ask for price |
Direct RT-PCR Kit wioRealStart DNA Polymerase |
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| FYT507-50P | Yeastern Biotech | 50 Preps | Ask for price |
PCR Tubes (50) |
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| PCR-50 | BioAssay Systems | 50 | EUR 15 |
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Description: To be used with the PractiChrom kits in conjuction with the PiCO Explorer. Quantity: 50 PCR tubes. |
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Allele-In-One Human Blood Direct PCR Kit |
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| ABP-PP-BD02100 | Allele Biotech | 100 RXNS | Ask for price |
Allele-In-One Human Blood Direct PCR Kit |
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| ABP-PP-BD02500 | Allele Biotech | 500 RXNS | Ask for price |
Allele-In-One Mouse Tail Direct PCR Kit |
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| ABP-PP-MT02100 | Allele Biotech | 100 RXNS | Ask for price |
Allele-In-One Mouse Tail Direct PCR Kit |
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| ABP-PP-MT02500 | Allele Biotech | 500 RXNS | Ask for price |
Direct RT-PCR Kit wi RealStart DNA Polymerase |
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| FYT506-100P | Yeastern Biotech | 100 Preps | Ask for price |
Direct RT-PCR Kit wi RealStart DNA Polymerase |
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| FYT506-50P | Yeastern Biotech | 50 Preps; | Ask for price |
MULTIPLEX KIT PCR MASTITIS PCR kit |
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| PCR-MPX218-48D | Bioingentech | 50T | EUR 543.6 |
MULTIPLEX KIT PCR MASTITIS PCR kit |
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| PCR-MPX218-96D | Bioingentech | 100T | EUR 686.4 |
MULTIPLEX KIT PCR Babesia & Theileria PCR kit |
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| PCR-MPX401-48D | Bioingentech | 50T | EUR 543.6 |
MULTIPLEX KIT PCR Babesia & Theileria PCR kit |
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| PCR-MPX401-96D | Bioingentech | 100T | EUR 686.4 |
ranavirus PCR kit |
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| PCR-V337-48D | Bioingentech | 50T | EUR 543.6 |
ranavirus PCR kit |
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| PCR-V337-96D | Bioingentech | 100T | EUR 686.4 |
Ruminococcus PCR kit |
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| PCR-V619-48D | Bioingentech | 50T | EUR 510.96 |
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Description: An conventional PCR kit for detection of Ruminococcus |
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Ruminococcus PCR kit |
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| PCR-V619-96D | Bioingentech | 100T | EUR 625.8 |
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Description: An conventional PCR kit for detection of Ruminococcus |
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Bifidobacterium PCR kit |
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| PCR-V621-48D | Bioingentech | 50T | EUR 510.96 |
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Description: An conventional PCR kit for detection of Bifidobacterium |
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Bifidobacterium PCR kit |
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| PCR-V621-96D | Bioingentech | 100T | EUR 625.8 |
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Description: An conventional PCR kit for detection of Bifidobacterium |
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Halobacillus PCR kit |
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| PCR-A635-48D | Bioingentech | 50T | EUR 543.6 |
Halobacillus PCR kit |
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| PCR-A635-96D | Bioingentech | 100T | EUR 686.4 |
Enterovirus PCR kit |
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| PCR-H433-48R | Bioingentech | 50T | EUR 987.6 |
Enterovirus PCR kit |
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| PCR-H433-96R | Bioingentech | 100T | EUR 1030.8 |
Mengovirus PCR kit |
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| PCR-H453-48R | Bioingentech | 50T | EUR 987.6 |
Mengovirus PCR kit |
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| PCR-H453-96R | Bioingentech | 100T | EUR 1335.6 |
Astrovirus PCR kit |
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| PCR-H491-48R | Bioingentech | 50T | EUR 987.6 |
Astrovirus PCR kit |
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| PCR-H491-96R | Bioingentech | 100T | EUR 1335.6 |
