Sars cov 2 lentivectors
Safely Study SARS-CoV-2 Interactions Designed to efficiently package most third-generation lentivectors, the pPACK-SPIKE SARS-CoV-2 “S” Pseudotype Lentivector Packaging Mix speeds up and simplifies the preparation of pseudotyped lentiviral particles with the SARS-CoV-2 Peak glycoprotein.
Based on the much-cited pPACKH1 packaging system from SBI but with a truncated SARS-CoV-2 spike protein1 replacing the standard VSV-G envelope protein, the pPACK-SPIKE packaging mix consists of three plasmids that produce all the structural and replication proteins necessary to transcribe and package an RNA copy of an expression lentivector into recombinant “Spike” pseudotyped lentiviral particles.
As a result, it can perform a variety of SARS-CoV-2 studies under BSL-2 conditions, including neutralization assays, studies of virus interactions with host surface proteins, and the development of vaccines and therapies.
For added convenience, we also offer pPACK-BALD, an envelope protein-free lentivector packaging mix that can be used as a negative control for pPACK-SPIKE studies or to create lentivirus particles pseudotyped with the envelope protein of your choice.
Also, you can compare this Spike protein from the original Wuhan-Hu-1 strain with the more prevalent Spike protein D614G (as of Summer 2020) with pPACK-SPIKE D614G.
Based on the popular and highly cited pPACKH1 packaging system from SBI Uses the codon-optimized SARS-CoV-2 protein “S” (the original Wuhan-Hu-1 strain) instead of the VSV-G envelope protein
It efficiently incorporates the Spike protein into pseudo viral particles due to a C-terminal truncation of 19 aa  Ideal for COVID-19 research, such as neutralization assays, under BSL2 conditions
Package any third-generation lentivector reporter of your choice, including popular LentiLabeler reporters from SBI
Available as a packed mix or packaged in a convenient kit that includes PureFection ™ Transfection Reagent and PEG-it Virus
Use with pPACK-BALD, a protein-free lentivector packaging system that is an ideal negative control Compared to Generalized D614G Spike Protein Using pPACK-SPIKE D614G
pPACK-SPIKE SARS-CoV-2 “S” Pseudotype Lentivector Packaging Mix is available as stand-alone packaging mixes with enough plasmids for 10 reactions (standard size) or 25 reactions (XL), as well as in a convenient kit format that includes Reagent PureFection ™ transfection solution and PEG-it virus precipitation solution.