anti c-myc 9E10
IMMUNIZING ANTIGEN Synthetic peptide corresponding to the carboxy-terminal of human c-myc : AEEQKLISEEDLL
SOURCE: Tissue culture supernatant
PURIFICATION METHOD: Protein A affinity chromatography
APPLICATIONS
*Epitope tagging on sequence EQKLISEEDL
*Western blotting ( 1/ 5000) of myc-tagged recombinant proteins and recombinant myc. Use under non reducing conditions. Detects a band of approximately 62 kDa (predicted molecular weight: 52.7 kDa).
*Immunohistochemical staining of formalin and fixed paraffin embedded tissue sections and also acetone fixed frozen sections
KNOWN SPECIES CROSS REACTIVITY Weakly cross-reactive with mouse but not rat
REFERENCES Evan, G.I., G.K. Lewis, G. Ramsay, and J. M. Bishop, Mol. Cell. Biol. 5, 3610-3616 (1985)Campbell, A. M., P. D. Kessler, and D.M. Farnborough J. Biol. Chem. 267, 9321-9325 (1992)Mikos, D., S. Caplan, D. Merens, G. Hynes, Z Pitluk, T. Kashi, K. Harrison-Lavoi, S. Stevenson, C. Brown, B. Barrell, A. L. Horwich, and K. Willison Proc. Natl. Acad. Sci. USA 91, 2743-2747 (1994)Gosney, J.R. J.K. Field, M.A. Gosney, M.D.W Lye, D.A. Spandidos and S. A. Butt Anticancer Res. 10, 623-628 (1990) Karayiannis, M., M. Yiagnisis, K. Papadimitriou, J.K. Field and D.A. Spandidos Anticancer Res. 10, 1127-1134 (1990)Spandidos, D.A., M. Yiagnisis, K. Papadimitriou, and J.K. Field. Anticancer Res. 9, 1385-1394 (1989)Roydsm J.A., M. Sharrard, B. Wagner, and S.V. Polacarz J. Pathol. 166, 225-233 (1992)Tiniakos, D., D.A. Spandidos, A. Kakkanas, A. Pintzas, L. Pollice, and G. Tiniakos, Anticancer Res. 9, 715-722 (1989)Tosca, A., S. Linardopoulos, A. Malliri, E. Hatziolou, A.Nicolaidou, and D. A. Spandidos, Anticancer Res. 11, 1433-1428 (1991)
PRESENTATION The monoclonal is presented at a concentration of 1 milligram in 1ml phosphate-buffered saline containing 10mM sodium azide We recommend that each laboratory determine an optimum working titer for use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.
Alternative names Cellular myelocytomatosis oncogene antibody, Myc protein antibody, Myc proto-oncogene protein antibody, c Myc antibody, C MYC antibody, v myc avian myelocytomatosis viral oncogene homolog antibody, v myc myelocytomatosis viral oncogene homolog (avian) antibody, Myc2 antibody, Niard antibody, Nird antibody
